Journal: Cell Reports Methods

Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome

doi: 10.1016/j.crmeth.2023.100455

Figure Lengend Snippet:

Article Snippet: GLUT3, Rabbit , Alomone , Cat# AGT-023; RRID:AB_2756644.

Techniques:

Journal: Alcohol and alcoholism (Oxford, Oxfordshire)

Article Title: Glycosylation is altered by ethanol in rat hippocampal cultured neurons.

doi: 10.1093/alcalc/agl044

Figure Lengend Snippet: Fig. 4. Western blot analysis of the glucose transporters GLUT1 and GLUT3 in control (CNT) and alcohol-exposed (EtOH) neurons. Histograms show increases in densitometric readings of GLUT1 and GLUT3 in alcohol-exposed cells compared with the controls. The data used for the statistical analysis are the mean ± SD of four independent experiments. Asterisks indicate significant differences (Student’s t-test P < 0.05).

Article Snippet: Antibodies: anti-MAP2 mouse monoclonal antibody (Sigma-Aldrich, Spain); anti-neurofilament (NF) 200 rabbit polyclonal antibody (Sigma-Aldrich); anti-GLUT1 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc. CA, USA), anti-GLUT3 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc.), anti-digoxigenin (DIG) mouse monoclonal antibody (Roche Diagnostics, S.L.

Techniques: Western Blot, Control

Journal: Molecular cancer therapeutics

Article Title: BRAF inhibition decreases cellular glucose uptake in melanoma in association with reduction in cell volume

doi: 10.1158/1535-7163.MCT-15-0080

Figure Lengend Snippet: Vemurafenib induces a decrease in transmembrane glucose uptake per cell. A, 3μM Vemurafenib (+) incubation induces a decrease in transport of the non-phosphorylatable glucose analog 3-OMG normalized to cell count in sensitive lines but not B, acquired resistant lines relative to DMSO (−) after 72 hours. C, Vemurafenib treatment does not cause a significant decrease in GLUT1 or GLUT3 proteins levels in sensitive or resistant lines by 72 hours. D, Vemurafenib does not induce a significant change in GLUT1 or GLUT3 mRNA levels after 72 hours as detected by RT-qPCR.

Article Snippet: Immunoblots were conducted with the following primary antibodies all used at 1:1000: Hexokinase II (cat. no. 2867; Cell Signaling Technology, Danvers, MA, USA), Beta Actin (cat. no. 4970; Cell Signaling), Beta Tubulin (cat. no. 2128; Cell Signaling), GSK3B (cat. no. 9315), p-GSK3B S9 (cat. no. 9323; Cell Signaling), p-p90RSK T573 (cat. no. 9346; Cell Signaling), RSK1/RSK2/RSK3 (cat. no. 9355; Cell Signaling), hsp60 (cat. no. sc-1052; Santa Cruz Biotechnology, Santa Cruz, CA, USA), GLUT1 (cat. no. 07-1401; Millipore, Billerica, MA, USA), GLUT3 (cat. no. NBP1-89762; Novus Biologicals, Littleton, CO, USA) and the secondary antibody Anti-rabbit IgG, HRP-linked Antibody (cat. no. 7074S; Cell Signaling).

Techniques: Incubation, Cell Counting, Quantitative RT-PCR